|
KMID : 0613820110210020251
|
|
Journal of Life Science
2011 Volume.21 No. 2 p.251 ~ p.256
|
|
|
EGCG induces Apoptosis under Hypoxic State in B16F10 Melanoma Cancer Cells
|
|
Kim In-Seop
Park Ock-Jin
Kim Young-Min
|
|
|
Abstract
|
|
|
|
EGCG, catechins in green tea, is a kind of phytochemical. Through the regulation of signal pathways, EGCG has been known to show anti-oxidant and anti-tumor effects in cells. In this study, we investigated the apoptotic effects of EGCG through AMP-activated protein kinase (AMPK) signal pathways, including hypoxia inducible factor-1 alpha (HIF-). The experiments were performed in B16F10 melanoma cells in a hypoxic state. AMPK is activated by ATP consumption such as nutrient deficiency, exercise, heat shock, etc. The activated AMPK that plays an important role as an energy sensor inhibits proliferation of cancer cells, as well as inducing apoptosis. HIF-, the primary transcriptional regulator of the response to oxygen deprivation, plays a critical role in modulating tumor growth and angiogenesis in a hypoxic state. The apoptotic effects of EGCG were studied in B16F10 cells in a hypoxic state. The results show that EGCG inhibits the transcriptional activity of HIF- and induces apoptosis. These observations suggest that EGCG may exert inhibitory effects of angiogenesis and control tumor cell growth in hypoxic melanoma cells.
|
|
|
KEYWORD
|
|
|
EGCG, AMP-activated protein kinase (AMPK), hypoxia inducible factor-1 alpha (HIF-), apoptosis, B16F10 melanoma cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|